Synergistic Bactericidal Activity of Silver Nanoparticles and Ciprofloxacin against Phytopathogens

The present study is to concentrate on the synergistic bactericidal effect of silver nanoparticles and ciproflaxacin against Pseudomonas solanocearum, Pseudomonas syringae, Xanthomonas malvacearum and Xanthomonas campestris. Silver nanoparticles were prepared in 0.1mM, 0.2 mM and 0.3 mM by Spirulina platensis. It exhibited anti microbial activity against all phytopathogens with varying degrees. The inhibition zone was 13mm, 18mm and 18mm at 0.1 mM, 0.2 mM and 0.3 mM respectively against Pseudomonas solanocearum. Pseudomonas syringae was comparatively less susceptible and exhibited 12mm, 16mm and 17mm inhibition zone at the same concentration. The antimicrobial activity was less in both Xanthomonas malvacearum and Xanthomonas campestris. When 0.2mM silver nanoparticles were used in combination with 1μg of ciprofloxacin the antiphytopathogenic activity was remarkably increased to 36mm, 40mm, 33mm and 35mm against all the Phytopathogens. Similarly MIC and MBC values were also decreased significantly showing the synergistic activity between silver nanoparticles and ciproflaxacin.